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تأثیر نانو ذرات سلنیوم بر فراسنجههای کمی و کیفی اسپرم سگ پس از نگهداری در یخچال | ||
علوم دامی ایران | ||
دوره 54، شماره 4، آذر 1402، صفحه 379-390 اصل مقاله (1.25 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijas.2023.350293.653914 | ||
نویسندگان | ||
مصطفی پناهی1؛ علی سلیمان زاده* 1؛ علی شالیزار جلالی2؛ اسماعیل آین1؛ رحیم مولایی3 | ||
1گروه مامایی و طیور، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. | ||
2گروه علوم پایه، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. | ||
3گروه بهداشت و مواد غذایی، دانشکده دامپزشکی، دانشگاه ارومیه، ارومیه، ایران. | ||
چکیده | ||
انجماد اسپرم به ابزاری ضروری در بیولوژی تولیدمثل تبدیلشده است. بااینحال، حفظ توان باروری در اسپرم منجمد شده دارای اهمیت بسیار بالایی است. هدف از مطالعه حاضر، بررسی اثرات نانو ذرات سلنیوم بر فراسنجههای کمی و کیفی منی سگ در دمای 4 درجه سانتیگراد در طی دوره 48 ساعته است. در این مطالعه، 20 انزال از 4 سگ نژاد میکس گرفته شد و در یک رقیقکننده بر پایه تریس رقیق شدند. سپس، به 4 قسمت در گروههای کنترل، و آزمایششده با 5/0، 1 و 5/1 میکروگرم نانو ذرات سلنیوم تقسیم شدند. فراسنجههای اسپرم شامل، ارزیابی تحرک کلی، حرکت پیشرونده اسپرم، فراسنجههای تحرک و قدرت زنده ماندن اسپرم به مدت 48 ساعت ارزیابی شد. نتایج بهدستآمده نشان داد که در غلظت 1 میکروگرم} تحرک کلی (27/1 ± 48/49) و پیشرونده اسپرم (48/1 ± 03/27) و قدرت زنده ماندن اسپرم (03/1 ± 40/41) { و غلظت 5/0 میکروگرم} تحرک کلی (54/1 ± 18/55) و پیشرونده اسپرم (67/1 ± 76/30) و قدرت زنده ماندن اسپرم (58/1 ± 15/43) { نانو ذرات سلنیوم در طی 48 ساعت ذخیرهسازی در مقایسه با گروه کنترل بهطور معنیداری (05/0>(p بالاتر بود، ولی در غلظت 5/1 میکروگرم کمتر از گروه کنترل بود (05/0>(p . درنتیجه، مطالعه حاضر نشان داد که افزودن نانو ذرات سلنیوم در غلظت 1 و 5/0 میکروگرم به منی سگ، میتواند فراسنجههای منی سگ را پس از ذخیرهسازی بهصورت مایع، بهبود بخشد اما در غلظت 5/1 باعث اثرات سمی شده و تحرک کلی و قدرت زنده ماندن اسپرم را کاهش داد. | ||
کلیدواژهها | ||
اسپرم؛ سگ؛ آنتیاکسیدان؛ نانو ذرات سلنیوم | ||
عنوان مقاله [English] | ||
Effect of selenium nanoparticles on quantitative and qualitative parameters of dog sperm after storage in the refrigerator | ||
نویسندگان [English] | ||
Mostafa Panahi1؛ Ali Soleimanzadeh1؛ Ali Shalizar-Jalali2؛ Esmail Ayen1؛ Rahim Molaie3 | ||
1Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. | ||
2Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. | ||
3Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. | ||
چکیده [English] | ||
Sperm cryopreservation has become an indispensable tool in reproductive biology. However, maintaining fertility in frozen sperm is very important. The aim of this study is to investigate the effects of selenium nano-particles on the storage of dog semen at 4 ˚C during a period of 48 hours. In this study, 20 ejaculates were collected from 4 mix dogs and diluted in a Tris-based diluent. Then, they were divided into 4 parts in control and treated with 0.5, 1 and 1.5 µg selenium nano-particles groups. Sperm parameters including evaluation of total and progressive motility sperm, motility characteristics and viability of sperm were evaluated for 48 hours. The obtained results showed that in concentrations of 1 µg [progressive sperm (27.03±1.48), total motility (49.48±1.27) and viability (41.40±1.03)] and 0.5 µg [progressive sperm (30.76±1.67), total motility (53.18±1.54) and viability (43.15±1.58)] of selenium nanoparticles compared to the control group, but in the concentration of 1.5 µg was lower than the control group (p< 0.05). As a result, the present study showed that adding selenium nanoparticles in concentrations of 0.1 and 0.5 µg to dog semen can improve the parameters of dog semen after liquid storage, but in concentrations of 1.5 µg it causes toxic effects and overall agitation. And it reduces sperm viability. | ||
کلیدواژهها [English] | ||
dog, sperm, Antioxidants, selenium nano-particles | ||
مراجع | ||
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