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بررسی اثر دما و سه زیرگونه از باکتری Bacillus thuringiensis بر تشکیل گره در سیستم ایمنی و برخی پارامتر‌های زیستی شب‌پره مدیترانه‌ای آرد Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| دوره 11، شماره 2، دی 1401، صفحه 135-152 اصل مقاله (1.43 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2024.372232.332 | ||
| نویسندگان | ||
| علیرضا میرزایی پارسا1؛ مریم راشکی* 1؛ الهام رضوان نژاد2 | ||
| 1گروه تنوع زیستی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران | ||
| 2گروه بیوتکنولوژی، پژوهشکده علوم محیطی، پژوهشگاه علوم و تکنولوژی پیشرفته و علوم محیطی، دانشگاه تحصیلات تکمیلی صنعتی و فناوری پیشرفته، کرمان، ایران | ||
| چکیده | ||
| باکتری بیمارگر حشرات،Bacillus thuringiensis ، گرم مثبت و اسپورزا است که تعداد زیادی از زیر‌گونه‌های آن شناسایی و معرفی‌شده‌است. پاسخ‌های ایمنی حشرات، پس از بلعیدن Bt یا پروتئین‌های سمی آن فعال‌می‌شود. هدف از این تحقیق، بررسی اثر دما و سه زیرگونة B. thuringiensis subspecies thuringiensis (Btt) ، B. thuringiensis subspecies aizawai (Bta) و B. thuringiensis subspecies galleriae (Btg) بر تشکیل گره طی پاسخ ایمنی و برخی پارامتر‌های زیستی شب‌پرة Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) بود. برای آلوده‌سازی بستر غذایی لارو، از غلظت 109 اسپور در میلیلیتر استفاده شد. این آزمایش در سه دمای مختلف (20، 25 و 30 درجة سلسیوس)، رطوبت نسبی 5±55 درصد و دورة نوری 16:8 (روشنایی: تاریکی) انجام شد. به‌طور معنیدار بیشترین تعداد گره تحت تأثیر زیر‌گونه‌های Bta و Btg در دمای 30 درجة‌سلسیوس و کمترین میزان گره در شاهد در هر سه دما مشاهده‌شد. مدت زمان تا شفیره‌شدن، در هر دما بین سه زیرگونه اختلاف معنیدار نداشت. اما، بین دماهای مختلف در هر زیرگونه اختلاف معنیدار مشاهده‌شد. همچنین، این مدت زمان در شاهد به‌طور معنیدار تحت تأثیر دما قرار‌گرفت. بیشترین میزان تولید شفیره مربوط به دماهای 20 و 25 درجة‌سلسیوس در زیرگونة Bta و شاهد بود که با هم اختلاف معنیدار نداشتند. تعداد حشرات کامل بدست‌آمده در تیمار Bta مشابه شاهد تحت تأثیر دما قرار نگرفت. از آنجا که دما بر تعداد گره‌ها در شاهد تأثیری نداشت، چنین استنباط می‌شود که افزایش دما با تأثیر بر باکتری Bt، تعداد گره‌های تشکیل‌شده را در لارو‌های زنده‌مانده افزایش داده و همچنین، نوع زیرگونة باکتری به‌طور معنیدار بر آن تأثیر داشته است. | ||
| کلیدواژه‌ها | ||
| اسپور؛ باکتری بیمارگر‌حشرات؛ تشکیل گره؛ دما؛ کنترل بیولوژیک | ||
| عنوان مقاله [English] | ||
| Investigating the impact of temperature and three subspecies of Bacillus thuringiensis on the nodulation of the immune system and some biological parameters of the Mediterranean flour moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae) | ||
| نویسندگان [English] | ||
| Alireza Mirzaei Parsa1؛ Maryam Rashki1؛ Elham Rezvannejad2 | ||
| 1Department of Biodiversity, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran | ||
| 2Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran | ||
| چکیده [English] | ||
| The entomopathogenic bacterium,Bacillus thuringiensis, is a gram-positive and spore-forming bacterium, and a large number of its subspecies have been identified and introduced. The insect immune responses activate after ingesting Bt or its toxin proteins. This research aimed to investigate the effect of temperature and three subspecies including B. thuringiensis subspecies thuringiensis (Btt), B. thuringiensis subspecies aizawai (Bta), and B. thuringiensis subspecies galleriae (Btg) on nodule formation and some biological parameters of the moth Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). A concentration of 109 spore/ml was used to contaminate the larval diet. This experiment was performed at three different temperatures (20, 25, and 30 ºC), relative humidity of 55±5%, and a photoperiod of 16:8 (L: D). The highest number of nodules was significantly observed in Bta and Btg treatments at 30 ºC and the lowest number of nodules was observed in the control. There was no significant difference in the time until pupation at any temperature among the three subspecies. However, a significant difference was observed between different temperatures in each subspecies. Therefore, this duration in the control was significantly affected by temperature. The highest amount of pupa production was related to the temperatures of 20 and 25 ºC in subspecies Bta and the control that had no significant difference. The number of adults obtained in the Bta treatment was not affected by the temperature, similar to the control. Since the temperature did not affect the number of nodules in the control, it is concluded that increasing the temperature by affecting the Bt increased the number of the nodules formed in the surviving larvae and also, the subspecies kind of the bacterium had a significant effect on it. | ||
| کلیدواژه‌ها [English] | ||
| Spore, entomopathogenic bacterium, nodulation, temperature, biological control | ||
| مراجع | ||
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