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Yavari Tehrani Fard, Negar, Amini, Zeynab, Aboutaleb, Nahid, Naseroleslami, Maryam. (1402). The Combined Effect of D-peptide B and Bifidobacterium bifidum Lysate on the Expression of MAP1LC3A and CASP3 Genes in Human Colorectal Adenocarcinoma Cells. , 34(4), 305-316. doi: 10.22059/jsciences.2024.363599.1007822
Negar Yavari Tehrani Fard; Zeynab Zeena Amini; Nahid Aboutaleb; Maryam Naseroleslami. "The Combined Effect of D-peptide B and Bifidobacterium bifidum Lysate on the Expression of MAP1LC3A and CASP3 Genes in Human Colorectal Adenocarcinoma Cells". , 34, 4, 1402, 305-316. doi: 10.22059/jsciences.2024.363599.1007822
Yavari Tehrani Fard, Negar, Amini, Zeynab, Aboutaleb, Nahid, Naseroleslami, Maryam. (1402). 'The Combined Effect of D-peptide B and Bifidobacterium bifidum Lysate on the Expression of MAP1LC3A and CASP3 Genes in Human Colorectal Adenocarcinoma Cells', , 34(4), pp. 305-316. doi: 10.22059/jsciences.2024.363599.1007822
Yavari Tehrani Fard, Negar, Amini, Zeynab, Aboutaleb, Nahid, Naseroleslami, Maryam. The Combined Effect of D-peptide B and Bifidobacterium bifidum Lysate on the Expression of MAP1LC3A and CASP3 Genes in Human Colorectal Adenocarcinoma Cells. , 1402; 34(4): 305-316. doi: 10.22059/jsciences.2024.363599.1007822

The Combined Effect of D-peptide B and Bifidobacterium bifidum Lysate on the Expression of MAP1LC3A and CASP3 Genes in Human Colorectal Adenocarcinoma Cells

Journal of Sciences, Islamic Republic of Iran
مقاله 1، دوره 34، شماره 4، اسفند 2023، صفحه 305-316    اصل مقاله (929.83 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22059/jsciences.2024.363599.1007822
نویسندگان
Negar Yavari Tehrani Fard1؛ Zeynab Zeena Amini2؛ Nahid Aboutaleb3؛ Maryam Naseroleslami* 1
11 Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Azad University of Medical Sciences, Tehran, Islamic Republic of Iran
22 College of Sport, Health and Engineering, Victoria University, Melbourne, Australia
33 Physiology Research Center, Iran University of Medical Sciences, Tehran, Islamic Republic of Iran
چکیده
In the realm of cancer research, novel approaches complementing conventional treatments are gaining prominence to improve patient outcomes. Antimicrobial peptides (AMPs), and bacteria, as emerging strategies, hold promise for cancer therapy. Recent studies suggested that the administration of peptides and bacteria may eliminate colorectal cancer (CRC) cells by triggering apoptosis, and autophagy processes, underscoring the potential of these compounds in CRC treatment. Using the human CRC cell line HT-29, we evaluated the combined effects of D-peptide B and Bifidobacterium bifidum lysate on the expression of the microtubule associated protein 1 light chain 3 alpha (MAP1LC3A), referred to as LC3A hereafter, and caspase 3 (CASP3) genes. D-peptide B and B. bifidum lysate were applied to control human embryonic kidney epithelial cell line HEK293 and HT-29 at their respective half-maximal inhibitory concentration (IC50) values. We performed total RNA extraction, complementary DNA (cDNA) synthesis, and reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) with gel electrophoresis to examine changes in the gene expression. Tukey's test and GraphPad Prism One-Way ANOVA were used for the statistical study. The combined treatment of D-peptide B and B. bifidum lysate with HT-29 cells resulted in a considerable decrease in the vitality of cancer cells. Furthermore, when HT-29 cells were compared to control cells, the expression of LC3A and CASP3 genes increased. The results point to the possibility of combining D-peptide B with B. bifidum lysate as a supplementary therapy for CRC. Furthermore, these findings may help improve our knowledge of the molecular processes driving apoptosis and autophagy, as well as their potential therapeutic implications in CRC.
کلیدواژه‌ها
Colorectal cancer؛ Antimicrobial peptides؛ Bifidobacterium bifidum؛ CASP3؛ LC3A
مراجع
  1. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians. 2018;68(6):394-424.
  2. Ahmed M. Colon cancer: a clinician’s perspective in 2019. Gastroenterology research. 2020;13(1):1.
  3. Ciardiello F, Ciardiello D, Martini G, Napolitano S, Tabernero J, Cervantes A. Clinical management of metastatic colorectal cancer in the era of precision medicine. CA: a cancer journal for clinicians. 2022;72(4):372-401.
  4. Higashi D, Futami K, Ishibashi Y, Egawa Y, Maekawa T, Matsui T, et al. Clinical course of colorectal cancer in patients with ulcerative colitis. Anticancer research. 2011;31(7):2499-504.
  5. Brown JP, Josse RG, Canada SACotOSo. 2002 clinical practice guidelines for the diagnosis and management of osteoporosis in Canada. Cmaj. 2002;167(10 suppl):S1-S34.
  6. Yockey M. Peptide Therapy. 2019.
  7. Hossain MS, Karuniawati H, Jairoun AA, Urbi Z, Ooi DJ, John A, et al. Colorectal cancer: a review of carcinogenesis, global epidemiology, current challenges, risk factors, preventive and treatment strategies. Cancers. 2022;14(7):1732.
  8. Hoskin DW, Ramamoorthy A. Studies on anticancer activities of antimicrobial peptides. Biochimica et Biophysica Acta (BBA)-Biomembranes. 2008;1778(2):357-75.
  9. Iwasaki T, Ishibashi J, Kubo M, Taylor D, Yamakawa M. Multiple functions of short synthetic enantiomeric peptides based on beetle defensins. Bioscience, biotechnology, and biochemistry. 2009;73(3):683-7.
  10. An BC, Ahn JY, Kwon D, Kwak SH, Heo JY, Kim S, et al. Anti-Cancer Roles of Probiotic-Derived P8 Protein in Colorectal Cancer Cell Line DLD-1. International Journal of Molecular Sciences. 2023;24(12):9857.
  11. Faghfoori Z, Faghfoori MH, Saber A, Izadi A, Yari Khosroushahi A. Anticancer effects of bifidobacteria on colon cancer cell lines. Cancer cell international. 2021;21(1):1-12.
  12. Xia C, Cai Y, Ren S, Xia C. Role of microbes in colorectal cancer therapy: Cross-talk between the microbiome and tumor microenvironment. Frontiers in Pharmacology. 2022;13:1051330.
  13. Sivamaruthi BS, Kesika P, Chaiyasut C. The role of probiotics in colorectal cancer management. Evidence-Based Complementary and Alternative Medicine. 2020;2020.
  14. Bloemberg D, Quadrilatero J. Autophagy, apoptosis, and mitochondria: molecular integration and physiological relevance in skeletal muscle. American Journal of Physiology-Cell Physiology. 2019;317(1):C111-C30.
  15. Iwasaki T, Ishibashi J, Tanaka H, Sato M, Asaoka A, Taylor D, et al. Selective cancer cell cytotoxicity of enantiomeric 9-mer peptides derived from beetle defensins depends on negatively charged phosphatidylserine on the cell surface. Peptides. 2009;30(4):660-8.
  16. Pfefferle PI, Prescott SL, Kopp M. Microbial influence on tolerance and opportunities for intervention with prebiotics/probiotics and bacterial lysates. Journal of allergy and clinical immunology. 2013;131(6):1453-63.
  17. Kim H, Kim HR, Kim N-R, Jeong BJ, Lee JS, Jang S, et al. Oral administration of Lactobacillus plantarum lysates attenuates the development of atopic dermatitis lesions in mouse models. Journal of Microbiology. 2015;53(1):47-52.
  18. Ganot N, Meker S, Reytman L, Tzubery A, Tshuva EY. Anticancer metal complexes: synthesis and cytotoxicity evaluation by the MTT assay. JoVE (Journal of Visualized Experiments). 2013(81):e50767.
  19. Gómez-Guillén MC, Montero MP. Enhancement of oral bioavailability of natural compounds and probiotics by mucoadhesive tailored biopolymer-based nanoparticles: A review. Food Hydrocolloids. 2021;118:106772.
  20. Eghtedari M, Porzani SJ, Nowruzi B. Anticancer potential of natural peptides from terrestrial and marine environments: A review. Phytochemistry Letters. 2021;42:87-103.
  21. Xiao Y-F, Jie M-M, Li B-S, Hu C-J, Xie R, Tang B, et al. Peptide-based treatment: a promising cancer therapy. Journal of immunology research. 2015;2015.
  22. Ehrenstein G, Lecar H. Electrically gated ionic channels in lipid bilayers. Quarterly reviews of biophysics. 1977;10(1):1-34.
  23. Peng F, Liao M, Qin R, Zhu S, Peng C, Fu L, et al. Regulated cell death (RCD) in cancer: key pathways and targeted therapies. Signal Transduction and Targeted Therapy. 2022;7(1):286.
  24. Maraming P, Klaynongsruang S, Boonsiri P, Maijaroen S, Daduang S, Chung JG, et al. Antitumor activity of RT2 peptide derived from crocodile leukocyte peptide on human colon cancer xenografts in nude mice. Environmental toxicology. 2018;33(9):972-7.
  25. Li X, Xia L, Ouyang X, Suyila Q, Su L, Su X. Bioactive peptides sensitize cells to anticancer effects of oxaliplatin in human colorectal cancer xenografts in nude mice. Protein and peptide letters. 2019;26(7):512-22.
  26. Dahan L, Sadok A, Formento JL, Seitz JF, Kovacic H. Modulation of cellular redox state underlies antagonism between oxaliplatin and cetuximab in human colorectal cancer cell lines. British journal of pharmacology. 2009;158(2):610-20.
  27. Tiptiri-Kourpeti A, Spyridopoulou K, Santarmaki V, Aindelis G, Tompoulidou E, Lamprianidou EE, et al. Lactobacillus casei exerts anti-proliferative effects accompanied by apoptotic cell death and up-regulation of TRAIL in colon carcinoma cells. PloS one. 2016;11(2):e0147960.
 
  1. Nowak A, Paliwoda A, Błasiak J. Anti-proliferative, pro-apoptotic and anti-oxidative activity of Lactobacillus and Bifidobacterium strains: A review of mechanisms and therapeutic perspectives. Critical reviews in food science and nutrition. 2019;59(21):3456-67.
  2. Kotzampassi K, Stavrou G, Damoraki G, Georgitsi M, Basdanis G, Tsaousi G, et al. A four-probiotics regimen reduces postoperative complications after colorectal surgery: a randomized, double-blind, placebo-controlled study. World journal of surgery. 2015;39(11):2776-83.
  3. Saber A, Alipour B, Faghfoori Z, Khosroushahi AY. Secretion metabolites of probiotic yeast, Pichia kudriavzevii AS-12, induces apoptosis pathways in human colorectal cancer cell lines. Nutrition research. 2017;41:36-46.
  4. You HJ, Oh D-K, Ji GE. Anticancerogenic effect of a novel chiroinositol-containing polysaccharide from Bifidobacterium bifidum BGN4. FEMS Microbiology letters. 2004;240(2):131-6.
  5. Ku S-M, You H-J, Ji G-E. Enhancement of anti-tumorigenic polysaccharide production, adhesion, and branch formation of Bifidobacterium bifidum BGN4 by phytic acid. Food Science and Biotechnology. 2009;18(3):749-54.
  6. Bahmani S, Azarpira N, Moazamian E. Anti-colon cancer activity of Bifidobacterium metabolites on colon cancer cell line SW742. The Turkish Journal of Gastroenterology. 2019;30(9):835.
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