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شناسایی جایگاههای ژنومی مرتبط با چندقلوزایی تحت انتخاب بین نژادهای گوسفندان بومی ایران و گوسفند نژاد رومانوف با عملکرد تولیدمثلی بالا | ||
علوم دامی ایران | ||
دوره 56، شماره 1، فروردین 1404، صفحه 1-18 اصل مقاله (1.76 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijas.2024.369473.653982 | ||
نویسندگان | ||
پرویز عزیزی1؛ محمد مرادی شهربابک* 2؛ حسین مرادی شهربابک3؛ مهدی مخبر4 | ||
1گروه علوم دامی، دانشکده کشاورزی، دانشگاه تهران، کرج. ایران | ||
2گروه علوم دامی دانشکده کشاورزی دانشگاه تهران. کرج. ایران | ||
3گروه علوم دامی، دانشکده کشاورزی ، دانشگاه تهران، کرج. ایران | ||
4گروه علوم دامی دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران. | ||
چکیده | ||
به منظور شناسایی ژنهای مرتبط با عملکرد تولیدمثلی تحت انتخاب بین نژادهای گوسفندان بومی ایران و نژاد رومانوف روسی از اطلاعات ژنومی 233 رأس گوسفند استفاده شد. دادههای مربوط به گوسفندان نژاد رومانوف از پایگاه اطلاعاتی iSheep و اطلاعات ژنومی گوسفندان ایرانی از پایگاه اطلاعاتی به آدرس https://disk.yandex.ru/d/3N2wEv0-9_NL0w، تهیه شدند. کنترل کیفی و غربالگری دادهها، آنالیز شاخص تمایز ژنتیکی(FST) و آنالیز مولفههای اصلی (PCA) جهت تعیین گروههای ژنتیکی با استفاده از نرم افزار PLINK1.9، صورت گرفت. متعاقب غربالگری نهایی صورت گرفته، اطلاعات ژنومی تعداد 488752 نشانگر SNP مشترک مربوط به کروموزومهای بدنی تعداد 79 رأس از نژاد رومانوف در مقابل تعداد 120 رأس مربوط به 9 نژاد از جمعیتهای دامی ایرانی برای جستجوی نشانههای انتخاب، مورد استفاده قرار گرفت. از برآوردگر نااریب FST (θ) و آمارهی XPEHH جهت کاوش نشانههای انتخاب مورد استفاده قرار گرفتند. استخراج ژنهای مرتبط با مناطق ژنومی تحت انتخاب توسط پایگاه اطلاعاتی آنلاین BIOMART با انطباق روی ژنوم گوسفند (Oar_v3.1) انجام شد. تعداد 489 ژن مرتبط با مناطق ژنومی تحت انتخاب (معادل 1/0 درصد کل نشانگرهای مورد مطالعه) شناسایی شدند. بررسی مناطق ژنومی تحت انتخاب نشان داد، از میان 489 ژن شناسایی شده مرتبط با تفاوتهای موجود بین دو گروه مورد مطالعه، تعداد 7 ژن درگیر در مسیرهای بیولوژیکی مرتبط با سنتز استروئید و استروئیدوژنز تخمدان بودند که بهعنوان موارد تحت انتخاب مرتبط با صفت چندقلوزایی، شناسایی شدند. شناسایی این مسیرهای بیولوژیکی و تلفیق آن با سایر اطلاعات مرتبط با صفت چندقلوزایی میتواند در طراحی برنامههای اصلاح نژادی جهت بهبود عملکرد تولیدمثلی، موثر باشد. | ||
کلیدواژهها | ||
جاروب انتخاب؛ آرایههای ژنومیکی؛ چندقلو زایی؛ گوسفندان بومی؛ نژاد رومانوف | ||
عنوان مقاله [English] | ||
Identification of genomic regions related to litter size involving divergent selection in Iranian indigenous and high reproductive Romanov sheep breeds | ||
نویسندگان [English] | ||
parviz Azizi1؛ Mohammad Moradi Shahrbabak2؛ Hossein Moradi Shahrbabak3؛ Mahdi Mokhber4 | ||
1Department of Animal Science, Faculty of Agriculture, Universityof Tehran, Karaj. Iran. | ||
2Department of Animal Science, Faculty of Agriculture, University of Tehran. Karaj. Iran | ||
3Department of Animal Science, Faculty of Agriculture, Universityof Tehran, Karaj. Iran. | ||
4Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia. Iran. | ||
چکیده [English] | ||
In order to identify genes related to reproductive performance under divergent selection in Iranian indigenous and high reproductive performance Romanov sheep breeds, the genomic data of 233 sheep were used. The genomeic data of Romanov sheep were obtained from the iSheep database and the genomic data of Iranian sheep was achieved from the database at https://disk.yandex.ru/d/3N2wEv0-9_NL0w. Data filtering and quality control, genetic differentiation index analysis (FST) and principal component analysis (PCA) were performed to determine genetic groups using PLINK 1.9 software. Following the final filtering, the genomic information of 488,752 common SNPs from autosomal chromosomes of 79 Romanov heads compared to 120 heads of nine Iranian indigenous sheep breeds to screening signature of selection. The ubiased FST (θ) estimator and XPEHH statistic were used to explore the signs of selection. The genes related to selected genomic regions were extracted using the BIOMART online database corresponding to areas in the sheep genome assembly (Oar 3.1). A total of 489 genes associated with the selected genomic regions (consisting of 0.1% of the studied markers) were identified. Investigation of the genomic regions under selection showed that out of all the identified genes related to the difference between the two studied groups, 7 genes were involved in biological pathways related to steroid synthesis and ovarian steroidogenesis that may be associated with litter size. Identifying this pathway and other complementary studies about genes involved in reproduction could be effective in designing breeding programs to improve reproductive performance. | ||
کلیدواژهها [English] | ||
Selective Sweep, Genomic array, Litter Size, Iranian native sheep, Romanov | ||
مراجع | ||
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