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Darbandi, Niloufar, komijani, Majid, Abdoli, Ali. (1403). Azithromycin Therapy Changed the Intestinal Microbiota, Caused Oxidative Stress, and Decreased Memory Function in Adult Wistar Rats. , 35(1), 5-14. doi: 10.22059/jsciences.2024.374944.1007858
Niloufar Darbandi; Majid komijani; Ali Abdoli. "Azithromycin Therapy Changed the Intestinal Microbiota, Caused Oxidative Stress, and Decreased Memory Function in Adult Wistar Rats". , 35, 1, 1403, 5-14. doi: 10.22059/jsciences.2024.374944.1007858
Darbandi, Niloufar, komijani, Majid, Abdoli, Ali. (1403). 'Azithromycin Therapy Changed the Intestinal Microbiota, Caused Oxidative Stress, and Decreased Memory Function in Adult Wistar Rats', , 35(1), pp. 5-14. doi: 10.22059/jsciences.2024.374944.1007858
Darbandi, Niloufar, komijani, Majid, Abdoli, Ali. Azithromycin Therapy Changed the Intestinal Microbiota, Caused Oxidative Stress, and Decreased Memory Function in Adult Wistar Rats. , 1403; 35(1): 5-14. doi: 10.22059/jsciences.2024.374944.1007858

Azithromycin Therapy Changed the Intestinal Microbiota, Caused Oxidative Stress, and Decreased Memory Function in Adult Wistar Rats

Journal of Sciences, Islamic Republic of Iran
مقاله 1، دوره 35، شماره 1، خرداد 2024، صفحه 5-14    اصل مقاله (494.7 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22059/jsciences.2024.374944.1007858
نویسندگان
Niloufar Darbandi* ؛ Majid komijani؛ Ali Abdoli
Department of Biology, Faculty of Sciencse, Arak University, Arak, 384817758, Islamic Republic of Iran
چکیده
Research has shown that the gut microbiome affects memory processes. Different antibiotic treatments lead to changes in the intestine microbiota and the dysbiosis caused by it is associated with changes in brain behavior. This study examined the effect of azithromycin on the intestine microbiome, oxidative stress, and memory in male Wistar rats. Two groups of adult male rats were established (n=16). In both groups, the first collection of the feces samples was done on the first day to identify the gut microbiome, then the animals were gavaged with normal saline or azithromycin (15 mg) in a volume of 1 ml daily for seven days. At the end of treatment, The second phase of collecting feces samples was completed. Then locomotion activity, novel object recognition test, Passive avoidance test, hippocampal neurons count and oxidative stress measurement in blood serum were performed. Azithromycin treatment induced a significant decrease in the number of aerobic and anaerobic colonies and led to the elimination of Enterococcus faecalis and Lactobacillus acidophilus species in the experimental group intestine. Azithromycin significantly decreased memory function, number of hippocampal healthy neurons, total antioxidant capacity, superoxide dismutase, and catalase enzymes and significantly increased the amount of malondialdehyde in blood serum compared to the control group. In this research, azithromycin by disrupting the intestinal microbiome, reduces diversity and suppresses some bacteria, raises levels of oxidative agents in blood serum, and by reducing the number of hippocampus-healthy neurons decreases cognitive functions.
کلیدواژه‌ها
Azithromycin Antibiotic؛ Gut Microbiome؛ Memory؛ Oxidative Stress؛ Rat
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