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Aldarwish, Mohammed, Aldosary, Naser, Alasadi, Ramiz, Alnajim, Ihab, Frhood, Aseel, Alasdib, Nidhal. (1403). Effect of Copper Sulfate and Zinc Oxide on the Growth of Vegetative Buds and Microbial Contamination in Date Palm Tissue Culture (cv. Barhi) in vitro. , 12(1), 157-166. doi: 10.22059/ijhst.2024.372925.787
Mohammed Aldarwish; Naser Aldosary; Ramiz M. Alasadi; Ihab Alnajim; Aseel A. Frhood; Nidhal Alasdib. "Effect of Copper Sulfate and Zinc Oxide on the Growth of Vegetative Buds and Microbial Contamination in Date Palm Tissue Culture (cv. Barhi) in vitro". , 12, 1, 1403, 157-166. doi: 10.22059/ijhst.2024.372925.787
Aldarwish, Mohammed, Aldosary, Naser, Alasadi, Ramiz, Alnajim, Ihab, Frhood, Aseel, Alasdib, Nidhal. (1403). 'Effect of Copper Sulfate and Zinc Oxide on the Growth of Vegetative Buds and Microbial Contamination in Date Palm Tissue Culture (cv. Barhi) in vitro', , 12(1), pp. 157-166. doi: 10.22059/ijhst.2024.372925.787
Aldarwish, Mohammed, Aldosary, Naser, Alasadi, Ramiz, Alnajim, Ihab, Frhood, Aseel, Alasdib, Nidhal. Effect of Copper Sulfate and Zinc Oxide on the Growth of Vegetative Buds and Microbial Contamination in Date Palm Tissue Culture (cv. Barhi) in vitro. , 1403; 12(1): 157-166. doi: 10.22059/ijhst.2024.372925.787

Effect of Copper Sulfate and Zinc Oxide on the Growth of Vegetative Buds and Microbial Contamination in Date Palm Tissue Culture (cv. Barhi) in vitro

International Journal of Horticultural Science and Technology
دوره 12، شماره 1، فروردین 2025، صفحه 157-166    اصل مقاله (705.94 K)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2024.372925.787
نویسندگان
Mohammed Aldarwish1؛ Naser Aldosary* 1؛ Ramiz M. Alasadi1؛ Ihab Alnajim1؛ Aseel A. Frhood1؛ Nidhal Alasdib2
1Date Palm Research Center, University of Basrah, Basra, Iraq
2College of Agriculture, University of Basrah, Basra, Iraq
چکیده
Zinc oxide and copper sulfate can assist in plant growth and development if they are added appropriately to plant nutrition. In plant tissue culture, microbial contamination negatively affects bud quality and causes numerous issues in plant regeneration. Adding zinc oxide and copper sulfate can enhance bud characteristics and inhibit microbial contamination. This research aimed to investigate the effects of copper sulfate and zinc oxide as microbicides to inhibit microbial contamination while embarking on in vitro plant tissue culture. The objective was to further assess the effects of both compounds on growth characteristics. Both compounds were added to the culture medium at various concentrations (0, 30, 60, and 90 mg L-1). Initial vegetative buds of the Barhi date palm variety were cultivated at six months of age. The results showed that zinc oxide was more effective than copper sulfate in stimulating plant vegetative growth and root development. In addition, higher concentrations of both compounds enhanced growth characteristics. Thus, zinc oxide and copper sulfate positively affected plant pigment content, mostly carotene. On the other hand, chlorophyll content was not significantly affected. Furthermore, zinc oxide was more effective than copper sulfate in reducing fungal and bacterial contamination, with the highest concentration (90 mg L1) yielding optimal results. These results suggested that zinc oxide and copper sulfate can effectively enhance the success rate of date palm tissue culture.  
کلیدواژه‌ها
Bud characteristics؛ Contamination؛ Copper sulfate؛ Tissue culture؛ Zinc oxide
آمار
تعداد مشاهده مقاله: 101
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