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اثربخشی نانوذرة نقره و اکسیتتراسایکلین در درمان آلودگی باکتریایی Aeromonas hydrophila در ماهی کپور معمولی (Cyprinus carpio) | ||
نشریه محیط زیست طبیعی | ||
دوره 78، ویژهنامه پایش و مدیریت آلایندههای محیطزیست طبیعی، تیر 1404، صفحه 75-85 اصل مقاله (754.19 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jne.2024.381255.2705 | ||
نویسندگان | ||
پگاه فرهنگ؛ امیررضا عابدعلم دوست* ؛ علیرضا میرواقفی | ||
گروه شیلات، دانشکدة منابع طبیعی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران. | ||
چکیده | ||
از متداولترین باکتریهای بیماریزا Aeromonas hydrophila است که باعث ایجاد انواع عفونت همراه با خونریزی در ماهیان پرورشی میگردد. راهکار عمومی، استفاده از انواع آنتیبیوتیک برای مقابله با این باکتری است. از طرفی، استفاده از فناوری نانوذرات با تکیه بر خواص آنتیباکتریایی، بهعنوان راهکاری مفید و جایگزین آنتیبیوتیکها مطرح است. بنابراین در این مطالعه کارایی آنتیبیوتیک اکسیتتراسایکلین 20% و نانوذرة نقره با اندازة 60-20 نانومتر در رفع بیماری حاصل از باکتری آئروموناسهیدروفیلا در ماهی کپور معمولی مد نظر قرارگرفته است. برای این منظور 5 تیمار شامل گروههای شاهد منفی، شاهد مثبت، بیمار با درمان اکسیتتراسایکلین، بیمار با درمان نانوذرة نقره و بیمار با درمان ترکیبی اکسیتتراسایکلین و نانوذرة نقره درنظر گرفته شد. مقدار مصرفی اکسیتتراسایکلین و نانوذرة نقره بهترتیب معادل36/0گرم و 0012/0 میلیگرم برآورد شد. براساس نتایج بهدستآمده میزان گلبولسفید در گروه کنترل مثبت در بیشترین مقدار (0/76±20/18) بوده و با دیگر تیمارها اختلاف (95/122F= ,00/0P=) دارد. همچنین میزان گلبولقرمز (38/18F= ,00/0P=) و هموگلوبین (43/12 F=,01 /0P=) در گروه کنترل منفی در بیشترین مقدار (79/0±40/ 8Hb= و 11/0±40/2RBC= ) و در گروه کنترل مثبت در کمترین مقدار (60/0±27/5Hb= و 26/0±38/1RBC=) بوده است. میزان درصد بقا در کمترین و بیشترین مقدار بهترتیب در گروه کنترل منفی (1/00±99/00) و گروه کنترل مثبت (52/2±33/21) مشاهده شد. بین افزایش گلبول قرمز، افزایش هموگلوبین، کاهش گلبول سفید و افزایش بقا همبستگی وجود دارد. بهطور کلی نانوذرة نقره و اکسیتتراسایکلین در کاهش علائم عفونی و مهارکنندگی باکتری نقش مؤثری دارند. استفادة همزمان از اکسیتتراسایکلین و نانونقره درصورتیکه با برنامهریزی مدون و مدیریتی، بهمنظور کاهش هزینههای درمان و خطرات محیطزیستی باشد، مناسب بهنظر میرسد. | ||
کلیدواژهها | ||
بقا؛ سلامت عمومی؛ سلولهای خونی؛ نانودارو؛ هموگلوبین | ||
عنوان مقاله [English] | ||
The efficacy of silver nanoparticles and oxytetracycline in treating Aeromonas hydrophila bacterial infection in common carp (Cyprinus carpio) | ||
نویسندگان [English] | ||
Pegah Farhang؛ Amirreza Abed-Elmdoust؛ Alireza Mirvaghefi | ||
Department of Fisheries, Faculty of Natural Resources, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. | ||
چکیده [English] | ||
One of the most common pathogenic bacteria is Aeromonas hydrophila, which causes various infections with bleeding in farmed fish. The general solution is to use different antibiotics to deal with this bacterium. On the other hand, nanoparticle technology based on antibacterial properties is considered a useful alternative to antibiotics. Therefore, in this study, the effectiveness of 20% oxytetracycline antibiotic and silver nanoparticles with a size of 20-60 nm in curing the disease caused by A. hydrophila bacteria in common carp fish has been considered. For this purpose, 5 treatments were considered: negative control, positive control, patient with oxytetracycline treatment, patient with silver nanoparticle treatment, and patient with combined treatment of oxytetracycline and silver nanoparticle. The consumption amount of oxytetracycline and silver nanoparticles was estimated as 0.36 g and 0.0012 mg, respectively. According to the results, the amount of white blood cells in the positive control group was the highest (20.18±0.76) and different from other treatments (P=0.00, F=122.95). Also, the number of red blood cells (P=0.00, F=18.38) and hemoglobin (P=0.01, F=12.43) in the negative control group was the highest (Hb=8.40±0.79 and RBC=2.40±0.11), and in the positive control group had the lowest value (Hb=5.27±0.60 and RBC=1.38±0.26). The lowest and highest survival percentage was observed in the negative control group (99.00±1.00) and the positive control group (21.33±2.52), respectively. There is a correlation between red blood cell increase, hemoglobin increase, white blood cell decrease, and increased survival. In general, silver nanoparticles and oxytetracycline play an effective role in reducing infectious symptoms and inhibiting bacteria. The simultaneous use of oxytetracycline and nanosilver seems appropriate if it is under-documented and management planning, to reduce treatment costs and environmental risks. | ||
کلیدواژهها [English] | ||
Blood cells, General health, Hemoglobin, Nanomedicine, Survival | ||
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