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غربالگری سریع باقیماندة اکسی‌تتراسایکلین (OTC) در فیلة ماهی قزل‌آلای رنگین‌کمان پرورشی (Oncorhynchus mykiss) با استفاده از آزمون الایزا | ||
| شیلات | ||
| دوره 77، شماره 4، آذر 1403، صفحه 269-280 اصل مقاله (1.06 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2024.379730.1439 | ||
| نویسندگان | ||
| رضوان موسوی ندوشن* 1؛ بهروز حضرتی2 | ||
| 1گروه شیلات، دانشکدة علوم و فنون دریایی, واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران. | ||
| 2گروه علوم و مهندسی صنایع غذایی، دانشکدة علوم و فنون دریایی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران. | ||
| چکیده | ||
| قزل‌آلای رنگین‌کمان یکی از محبوب‌ترین گونة‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌‌های آزاد ماهیان در جهان است. افزایش بازار تقاضا برای این گونه منجر به توسعة پرورش متراکم، شیوع عفونت‌های میکروبی و مصرف آنتی‌بیوتیک‌ها در دورة پرورش شده است. یک گروه از آنتی‌بیوتیک‌های پرمصرف تتراسیکلین‌ها به‌ویژه اکسی‌تتراسایکلین (OTC) است. در این تحقیق نمونه‌های ماهی قزل‌آلا از مزارع منطقة پلور، کرج، دماوند (عمدة توزیع در استان تهران)، سراب و سیوان-صوفیان (عمده توزیع و بازار مصرف: استان آذربایجان شرقی) خریداری و 30 نمونه از هر مزرعه و در مجموع 300 ماهی غربالگری شدند. محدودة تشخیص برای OTC در قزل‌آلای رنگین‌کمان 0/3 نانوگرم در گرم و واکنش متقابل با آنتی‌بادی 100درصد به‌دست آمد. نتایج نشان داد کلیة ماهیان قزل‌آلا از نظر OTC مثبت بوده، میانگین باقیماندة کسی‌تتراسایکلین کمتر از حداکثر حد مجاز ppb) 2/0MRLs: ) در Codex Alimentarius بودند. این در حالی است که حد مجاز برای OTC در اتحادیه اروپا 1/0 نانوگرم در گرم تعیین شده است. بر این اساس مصرف قزل‌آلا از مزارع استان آذربایجان شرقی و دماوند ایمن، اما مصرف قزل‌آلای تهیه شده از مزارع کرج و پلور (ppb 130-145) برای افراد حساس و پرخطر توصیه نمی‌شود. در نهایت، استفاده از OTC نباید افزایش یابد تا همچنان در صنعت آبزی‌پروری در سطح مناسب باقی بماند. به‌عنوان آخرین نکته، وجود مقادیر بالای باقیماندة OTC در منطقة کرج و پلور مشخص می‌کند که تتراسایکلین در کل دورة پرورش و همراه با رژیم غذایی روزانه مورد استفاده قرار می‌گیرد و همچنین دوره قطع مصرف جهت حذف، قبل از جمع‌آوری و عرضة ماهیان به بازار مصرف رعایت نمی‌شود. | ||
| کلیدواژه‌ها | ||
| باقیماندةاکسی‌تتراسایکلین؛ الایزا؛ قزل‌آلای رنگین‌کمان؛ ماهی پرورشی | ||
| عنوان مقاله [English] | ||
| Quick screening of Oxytetracycline (OTC) residue in cultured rainbow trout (Oncorhynchus mykiss) fillet using ELISA assay | ||
| نویسندگان [English] | ||
| Rezvan Mousavi Nadushan1؛ Behruz Hazrati2 | ||
| 1Department of Fishery, Faculty of Marine science and Technology, Tehran North Branch, Islamic Azad University Tehran, Tehran, Iran. | ||
| 2Department of Food Science & Technology, Faculty of Marine Science and Technology, Tehran North Branch, Islamic Azad University Tehran, Tehran, Iran. | ||
| چکیده [English] | ||
| Rainbow trout is one of the most popular salmonid fish in the world. Increased market demand for rainbow trout has led to the development of intensive farming and the spread of microbial infections during the rearing period. A group of widely used antibiotics is tetracycline, especially oxytetracycline (OTC). In this research, trout samples were purchased from farms in Polur, Karaj, Damavand (main distribution in Tehran province), Sarab and Sivan-Sufian (consumer market: East Azerbaijan province) and 30 samples from each farm and a total of 300 fish were screened. The detection limit for OTC in rainbow trout was 0.3 ppb and the antibody cross-reactivity was 100%. All salmon were positive for OTC, the mean OTC residues were below the Maximum Limits (MRLs) in Codex Alimentarius. While the limit for OTC in the European Union is set at 0.1 ppm, the consumption of trout from the farms of East Azarbaijan and Damavand is safe, but the consumption of trout prepared from Karaj and Polur farms (ppb 145-130) is not recommended for sensitive and high-risk people. Finally, OTC use should not increase to remain at an appropriate level in the fisheries industry. As a last point, the presence of high amounts of OTC residues in Karaj and Pleur region indicates that tetracycline is consumed during the entire rearing period, along with the daily diet, as well as the withdrawal period for removal, before collecting and sending the fishes to the market is not complied. | ||
| کلیدواژه‌ها [English] | ||
| Cultured fish, ELISA, Oxytetracycline residue, rainbow trout | ||
| مراجع | ||
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