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Ghanbari Jahromi, Marziea, Rahnama, Hassan, Mousavi, Amir, Safarnejad, Mohammad Reza, Kalatejari, Sepide, Soheilivand, Saeed. (1394). Transient expression of coding and non-coding regions of PVY confer resistance to virus infection. , 5(1), 19-31. doi: 10.22059/pbs.2015.53952
Marziea Ghanbari Jahromi; Hassan Rahnama; Amir Mousavi; Mohammad Reza Safarnejad; Sepide Kalatejari; Saeed Soheilivand. "Transient expression of coding and non-coding regions of PVY confer resistance to virus infection". , 5, 1, 1394, 19-31. doi: 10.22059/pbs.2015.53952
Ghanbari Jahromi, Marziea, Rahnama, Hassan, Mousavi, Amir, Safarnejad, Mohammad Reza, Kalatejari, Sepide, Soheilivand, Saeed. (1394). 'Transient expression of coding and non-coding regions of PVY confer resistance to virus infection', , 5(1), pp. 19-31. doi: 10.22059/pbs.2015.53952
Ghanbari Jahromi, Marziea, Rahnama, Hassan, Mousavi, Amir, Safarnejad, Mohammad Reza, Kalatejari, Sepide, Soheilivand, Saeed. Transient expression of coding and non-coding regions of PVY confer resistance to virus infection. , 1394; 5(1): 19-31. doi: 10.22059/pbs.2015.53952

Transient expression of coding and non-coding regions of PVY confer resistance to virus infection

Progress in Biological Sciences
مقاله 2، دوره 5، شماره 1، شهریور 2015، صفحه 19-31    اصل مقاله (481.65 K)
نوع مقاله: Original Research Papers
شناسه دیجیتال (DOI): 10.22059/pbs.2015.53952
نویسندگان
Marziea Ghanbari Jahromi* 1؛ Hassan Rahnama2؛ Amir Mousavi3؛ Mohammad Reza Safarnejad4؛ Sepide Kalatejari1؛ Saeed Soheilivand2
1Department of Horticultural Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, Iran
3Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
4Department of Plant Viruses, Iranian Research Institute of Plant Protection, Tehran, Iran
چکیده
One of the most efficient mechanisms by which plants protect themselves from invading viruses
is the specific RNA-dependent silencing pathway termed post-transcriptional gene silencing
(PTGS). In this mechanism, resistance to a virus is engineered through the expression of a
segment of the virus genomein transgenic plants. Potato VirusY (PVY) is one of the most
damaging viruses of potato, infecting most cultivars and causing significant yield losses
throughout the world. The present study was performed to compare the efficiency of three
construct containing different regions of 3′UTR (UR) and coat protein (CP) against PVY
infection. Expression of homologous hairpin RNA to PVY in potato plant was carried out by
transient gene expression of constructs with agro-infiltration followed by mechanical viral
infection. Results showed that successful production of siRNAs confer resistance to two PVY
strain. Comparison between transiently expressed constructs indicated that applying CP+UR
PVY hairpin RNA was the most efficient RNAi construct to confer resistance. Resistance was
found to have taken the form of immunity, since no viral particle could be detected in the upper
leaves as shown by ELISA assay and Northern hybridizations. To the best of our knowledge,
this is the first report on the application of 3'non-coding region of PVY in conferring complete
resistance against virus in potato.
کلیدواژه‌ها
potato؛ PVY؛ RNA silencing؛ transient expression
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