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جمالی, فاطمه, بیات, فرشته. (1394). مطالعه فنوتیپی و ژنوتیپی Pseudomonas fluorescens جدایه PGU0 و ارزیابی توانایی بیوکنترلی آن علیه Rhizoctonia solani عامل مرگ گیاهچه لوبیا. , 4(1), 37-46. doi: 10.22059/jbioc.2015.54875
فاطمه جمالی; فرشته بیات. "مطالعه فنوتیپی و ژنوتیپی Pseudomonas fluorescens جدایه PGU0 و ارزیابی توانایی بیوکنترلی آن علیه Rhizoctonia solani عامل مرگ گیاهچه لوبیا". , 4, 1, 1394, 37-46. doi: 10.22059/jbioc.2015.54875
جمالی, فاطمه, بیات, فرشته. (1394). 'مطالعه فنوتیپی و ژنوتیپی Pseudomonas fluorescens جدایه PGU0 و ارزیابی توانایی بیوکنترلی آن علیه Rhizoctonia solani عامل مرگ گیاهچه لوبیا', , 4(1), pp. 37-46. doi: 10.22059/jbioc.2015.54875
جمالی, فاطمه, بیات, فرشته. مطالعه فنوتیپی و ژنوتیپی Pseudomonas fluorescens جدایه PGU0 و ارزیابی توانایی بیوکنترلی آن علیه Rhizoctonia solani عامل مرگ گیاهچه لوبیا. , 1394; 4(1): 37-46. doi: 10.22059/jbioc.2015.54875

مطالعه فنوتیپی و ژنوتیپی Pseudomonas fluorescens جدایه PGU0 و ارزیابی توانایی بیوکنترلی آن علیه Rhizoctonia solani عامل مرگ گیاهچه لوبیا

کنترل بیولوژیک آفات و بیماری های گیاهی
مقاله 5، دوره 4، شماره 1، فروردین 1394، صفحه 37-46    اصل مقاله (676.89 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22059/jbioc.2015.54875
نویسندگان
فاطمه جمالی* ؛ فرشته بیات
استادیار گروه اصلاح نباتات، دانشکدة کشاورزی و منابع طبیعی، دانشگاه خلیج‌فارس بوشهر
چکیده
سودوموناس‌های فلورسنت مولد آنتی‌بیوتیک 2 و 4ـ دی استیل فلورو گلوسینول(DAPG)  برای استفاده به‌عنوان عوامل بیوکنترل، در سراسر دنیا، به‌خوبی مطالعه شده‌اند. استرین Pseudomonas fluorescens PGU0 از ریزوسفر آفتابگردان در استان بوشهر جداسازی شد. آزمون کشت متقابل مشخص کرد که این استرین در شرایط درون‌شیشه‌ای فعالیت آنتاگونیستی علیه قارچ Rhizoctonia solani روی محیط‌های کشت مختلف را دارد و در شرایط اتاقک رشد نیز موجب کاهش شدت بیماری (52 درصد) و افزایش وزن خشک گیاهچه‌های آلودة لوبیا (80 درصد) می‌شود. واکنش زنجیره‌ای پلیمراز با پرایمرهای اختصاصی، وجود ژن‌های کلیدی (phlD، hcnBC و pltB) دخیل در بیوسنتز متابولیت‌های ضد میکروبی، به‌ترتیب،Phl ، پایولوتورین (PLT) و سیانید ‌هیدروژن (HCN) را مشخص کرد. تولید آنتی‌بیوتیک‌ها با کروماتوگرافی مایع با کارایی بالا (HPLC) بررسی و مشخص شد این باکتری توانایی تولید آنتی‌بیوتیک‌های DAPG و PLT را دارد. همچنین، تولید سیانید ‌هیدروژن و سیدروفور در شرایط آزمایشگاهی توسط این باکتری به اثبات رسید. آنالیز فیلوژنتیکی 16S rDNA شباهت این استرین را با سایر استرین‌های بیوکنترل مهم دنیا اثبات کرد که می‌توانند متابولیت‌های ضد میکروبی نظیر Phl و Plt را تولید کنند.
کلیدواژه‌ها
بیوکنترل؛ پایولوتورین؛ سیانید ‌هیدروژن؛ 2 و 4 ـ دی استیل فلورو گلوسینول
عنوان مقاله [English]
Phenotypic and genotypic study of Pseudomonas fluorescens strains PGU0 and assessment of its biocontrol against Rhizoctonia solani, the causal agent of bean damping- off
نویسندگان [English]
Fatemeh Jamali؛ Fereshteh Bayat
Assistant Professors, Plant Breeding Department, College of Agriculture and Natural resources, Persian Gulf University, Booshehr, Iran
چکیده [English]
Fluorescent pseudomonads producing 2,4-diacetylphloroglucinol (DAPG) antibiotic have been well studied for use as biocontrol agents around the world. Pseudomonas fluorescens PGU0 strain was isolated from the rhizosphere of sunflower in Bushehr Province. Dual culture test showed that the strain had antagonistic activity against Rhizoctonia solani fungus on different media cultures; moreover, under growth chamber condition, the strain reduced disease severity by 52% and caused an increase in dry weight of infected bean seedlings by 80%. The polymerase chain reaction (PCR) using specific primers, demonstrated the presence of key genes (phlD, hcnBC and pltB) involved in the biosynthesis of antimicrobial metabolites Phl, Pyoluteorin (PLT) and hydrogen cyanide (HCN), respectively . Antibiotic production was examined by high-performance liquid chromatography (HPLC) and it was found that these bacteria are capable of producing DAPG and PLT antibiotics. On the other hand, production of hydrogen cyanide and siderophore by the bacterium was demonstrated under lab conditions. Phylogenetic analysis of 16S rDNA proved similarity of this strain with other world biocontrol strains capable of producing antimicrobial metabolites like Phl and Plt.
کلیدواژه‌ها [English]
Biocontrol, 2, 4-diacetylphloroglucinol, pyoluteorin, hydrogen cyanide
مراجع
  1. Ahmadzadeh M (2001) Influence of antagonistic rhizobacteria of Pseudomonas and Bacillus genus against seed rot and damping-off diseases of beans and studying their antagonistic mechanisms. Ph.D. thesis in Plant pathology, College of Agriculture, Tehran University. (In Persian)
  2. Alstrom S (1989) Factors associated with detrimental effects of rhizobacteria on plant growth. Plant and Soil 102: 3-9.
  3. Behboudi K (2004)Influence of fluorescent pseudomonads on Sclerotinia sclerotiorum and S. minor and their tolerance to 2,4-diacetylphloroglucinol gene expression in sunflower rhizosphere. Ph.D. thesis in Plant Pathology, College of Agriculture, Tehran University. (In Persian)
  4. Benizri E, Courtade A, Picard C, Guckert A (1998) Role of maize root exudates in the production of auxins by Pseudomonas fluorescens M.3.1. Soil Biology and Biochemistry 30: 1481-1484.
  5. Bonsall RF, Weller DM, Thomashow LS (1997) Quantification of 2,4-diacetylphloroglucinol produced by fluorescent Pseudomonas spp. in vitro and in the rhizosphere of wheat. Applied Environmental Microbiology 63: 951–955
  6. Bowden K, Broadbent JL, Ross WJ (1965) Some simple anthelmintics. British Journal of Pharmacology 24: 714–724.
  7. Castaneda GC, Munoz TJJ, Videa JRP (2005) A spectrophotometric method to determine the siderophore production by strains of fluorescent Pseudomonas in the presence of copper and iron. Microchemical Journal 81: 35-40.
  8. Cronin D, Moënne-Loccoz Y, Fenton A, Dunne C, Dowling DN, O’Gara F (1997). Role of 2,4-diacetylphloroglucinol in the interactions of the biocontrol pseudomonad F113 with the potato cyst nematode Globodera rostochiensis. Applied Environmental Microbiology 63: 1357-1361.
  9. De La Fuente L, Thomashow L, Weller D, Bajsa N, Quagliotto L, Chernin L, Arias A (2004) Pseudomonas fluorescens UP61 isolated from birdsfoot trefoil rhizosphere produces multiple antibiotics and exerts a broad spectrum of biocontrol activity. European Journal of Plant Pathology 110:671-681.
  10. Duffy BK, Défago G (1999) Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Applied Environmental Microbiology 65(6): 2429-2438.
  11. Fenton AM, Stephens PM, Crowley J, OۥCallaghan M, OۥGara F (1992) Exploitation of gene(s) in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a Pseudomonas strain. Applied Environmental Microbiology 58: 3873-3878.
  12. Frapolli M, Défago G, Möenne-Loccoz M (2007) Multilocus sequence analysis of biocontrol fluorescent Pseudomonas spp. producing the antifungal compound 2,4-diacetylphloroglucinol. Environmental Microbiology 9: 1939-1955.
  13. Gould WD, Hagedron C, Bradinelli TR, Zablatowics RM (1985) New selective media for enumeration and recovery of fluorescent pseudomonads from various habitats. Applied Environmental Microbiology 49: 28-32.
  14. Haas D, Défago G (2005) Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature Reviews Microbiology 3: 307-319.
  15. Handelsman J, Stabb EV (1996) Biocontrol of soilborne plant pathogens. Plant Cell 8: 1855–1869.
  16. Jamali F (2009a) Influence of some biotic factors on the expression of hydrogen cyanide- and 2,4-diacetylphloroglucinol biosynthesis genes in Pseudomonas fluorescens on bean rhizosphere, Ph. D. thesis in Plant pathology, College of Agriculture, Tehran University. (In Persian)
  17. Jamali F, Sharifi-Tehrani A, Lutz MP Maurhofer M (2009b) Influence of host plant genotype, presence of a pathogen, and coinoculation with Pseudomonas fluorescens strans on the expression of hydrogen cyanide and 2,4-diacetylphloroglucinol biosynthetic genes in P. fluorescens strain CHA0. Microbial Ecology 57(2): 267-275.
  18. Keel C, Schnider U, Maurhofer M, Voisard C, Laville J, Burger U, Wirthner P, Haas D, Défago G (1992) Suppression of root diseases by Pseudomonas fluorescens CHA0: Importance of the bacterial secondary metabolite 2,4-diacetylphloroglucinol. Molecular Plant-Microbe Interaction 5: 4-13.
  19. Keel C, Weller DM, Natsch A, Défago G, Cook RJ, Thomashow LS (1996) Conservation of the 2,4-diacetylphloroglucinol biosynthesis locus among fluorescent Pseudomonas strains from diverse geographic locations. Applied Environmental Microbiology 62: 552-563.
  20. King EO, Ward MK, Rainey DE (1954) Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44: 301-307.
  21. Kloepper JW, Tuzun S, Liu L Wei G (1993) Plant growth promoting rhizobacteria as inducers of systemic disease resistance. In: Lumsden RD, Vaughn JL (eds), Pest Management: Biologically Based Technologies, American Chemical Society Books, Washington, DC, USA. pp. 156–165.
  22. Kraus J, Loper JE (1995) Characterization of a genomic region required for production of the antibiotic pyoluteorin by the biological control agent Pseudomonas fluorescens Pf-5. Applied Environmental Microbiology 61: 849-854.
  23. Landa BB, Mavrodi DM, Raaijmakers JM, McSpadden Gardener BB, Thomashow LS, Weller DM (2002) Differential ability of genotypes of 2,4-diacetylphloroglucinol-producing Pseudomonas fluorescens strains to colonize the roots of pea plants. Applied Environmental Microbiology 68: 3226-3237.
  24. Maurhofer M, Keel C, Défago G (1995) Influence of plant species on disease suppression by Pseudomonas fluorescens strain CHA0 with enhanced antibiotic production. Plant  Pathology  44: 40-50.
  25. Maurhofer M, Keel C, Haas D, Défago G (1994) Pyoluteorin production by Pseudomonas fluorescens strain CHA0 is involved in the suppression of Pythium damping-off of cress, but not cucumber. European Journal of Plant Pathology100: 221-232.
  26. Mavrodi OV, McSpadden Gardener BB, Mavrodi DV, Bonsall RF, Weller DM, Thomashow L S (2001) Genetic diversity of phlD from 2,4-diacetylphloroglucinol-producing fluorescent Pseudomonas spp. Phytopathology 91: 35-43.
  27. Mc Spadden Gardener BB, Mavrodi DV, Thomashow LS, Weller DM (2001) A rapid polymerase chain reaction-based assay characterizing rhizosphere populations of 2,4-diacetylphloroglucinol-producing bacteria. Phytopathology 91: 44-54.
  28. Mc Spadden Gardener BB, Schroeder KL, Kalloger SE (2000) Genotypic and phenotypic diversity of phlD-containing Pseodomonas strains isolated from the rhizosphere of wheat. Applied Environmental Microbiology 66: 1939-1946.
  29. Nowak-Thompson B, Chaney N, Wing JS, Gould SJ and Loper JE (1999) Characterization  of the pyoluteorin  biosynthetic gene cluster  of Pseudomonas  fluorescens Pf-5.  Journal of Bacteriology 181: 2166–2174.
  30. Raaijmakers JM, Weller DM, Thomashow LS (1997) Frequency of antibiotic-producing Pseudomonas spp. in natural environments. Applied Environmental Microbiology 63: 881-887.
  31. Sharifi-Tehrani, A., Zala, M., Natsch, A., Moënne-Loccoz, Y., and Défago, G. 1998. Biocontrol of soil-borne fungal plant diseases by 2,4-diacetylphloroglucinol-producing fluorescent pseudomonads with different restriction profiles of amplified 16S rDNA. Eur. J. Plant Pathol. 104: 631-643.
  32. Siddiqui, I. A., and Shaukat, S. S. 2002. Resistance against the damping-off fungus Rhizoctonia solani systemically induced by the plant-growth-promoting rhizobacteria Pseudomonas aeruginosa (IE-6S+) and P. fluorescens (CHA0). Journal of Phytopathology. 150: 550-506.
  1. Sperber JI (1958) The incidence of apatite-solubilizing organisms in the rhizosphere and soil. Australian Journal of Agricultural Research 9: 778-781.
  1. Svercel M, Duffy B, Défago G (2007) PCR amplification of hydrogen cyanide biosynthetic locus hcnAB in Pseudomonas spp. Journal of Microbiological Methods 70: 209-213.
  2. Thomashow LS, Weller DM (1996) Current concepts in the use of introduced bacteria for biological control: mechanisms and antifungal metabolites, In: Stacey G, Keen NT (eds.), Plant-Microbe Interactions, Vol. 1. Chapman and Hall, New York. pp. 187-235.
  3. Voisard C, Bull CT, Keel C, Laville J, Maurhofer M, Schnider U, De´fago G, Haas, D (1994) Biocontrol of root diseases by Pseudomonas fluorescens CHA0: Current concepts and experimental approaches. In: O’Gara F, Dowling DN, Boesten B (eds), Molecular Ecology of Rhizosphere Microorganisms: Biotechnology and the Release of GMOs. VCH Verlagsgesllschaft mbH, Weinheim, Germany. pp. 67– 89.
  4. Wang C, Ramette A, Pungasamarnwong P, Zala M, Natsch A, Moënne-Loccoz Y, Défago G (2001) Cosmopolitan distribution of phlD-containing dicotyledonous crop-associated biocontrol pseudomonads of worldwide origin. FEMS Microbiology Ecology 37: 105-116.
  5. Weller DM (2007) Pseudomonas biocontrol agents of soilborne pathogens: looking back over 30 years. Phytopathology 97: 250-256.
  6. Weller DM, Raaijmakers JM, McSpadden Gardener BB, Thomashow LS (2002) Microbial populations responsible for specific suppressiveness to plant pathogens. Annual Review of. Phytopathology 40: 309-348.
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