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مخبر, مهدی, مرادی شهربابک, محمد, صادقی, مصطفی, مرادی شهربابک, حسین, ویلیامز, جان. (1394). پویش ژنومی نشانه‌های انتخاب در گاومیش‌های خوزستانی و مازندرانی. , 46(2), 119-131. doi: 10.22059/ijas.2015.55643
مهدی مخبر; محمد مرادی شهربابک; مصطفی صادقی; حسین مرادی شهربابک; جان ویلیامز. "پویش ژنومی نشانه‌های انتخاب در گاومیش‌های خوزستانی و مازندرانی". , 46, 2, 1394, 119-131. doi: 10.22059/ijas.2015.55643
مخبر, مهدی, مرادی شهربابک, محمد, صادقی, مصطفی, مرادی شهربابک, حسین, ویلیامز, جان. (1394). 'پویش ژنومی نشانه‌های انتخاب در گاومیش‌های خوزستانی و مازندرانی', , 46(2), pp. 119-131. doi: 10.22059/ijas.2015.55643
مخبر, مهدی, مرادی شهربابک, محمد, صادقی, مصطفی, مرادی شهربابک, حسین, ویلیامز, جان. پویش ژنومی نشانه‌های انتخاب در گاومیش‌های خوزستانی و مازندرانی. , 1394; 46(2): 119-131. doi: 10.22059/ijas.2015.55643

پویش ژنومی نشانه‌های انتخاب در گاومیش‌های خوزستانی و مازندرانی

علوم دامی ایران
مقاله 3، دوره 46، شماره 2، تیر 1394، صفحه 119-131    اصل مقاله (745.78 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22059/ijas.2015.55643
نویسندگان
مهدی مخبر1؛ محمد مرادی شهربابک* 2؛ مصطفی صادقی3؛ حسین مرادی شهربابک3؛ جان ویلیامز4
1دانشجوی دکتری ژنتیک و اصلاح نژاد، گروه مهندسی علوم دامی دانشگاه تهران، کرج، ایران
2استاد گروه مهندسی علوم دامی دانشگاه تهران، کرج، ایران
3استادیار گروه مهندسی علوم دامی دانشگاه تهران، کرج، ایران
4استاد و مدیر بخش ژنومیک مرکز تحقیقات Padano، ایتالیا
چکیده
شناسایی مناطق ژنومی که هدف انتخاب بوده‌اند، از بحث‌برانگیزترین مباحث در حوزة تحقیقات ژنتیک حیوانی، به‌خصوص در حیوانات اهلی است. در این مطالعه، پویش کل ژنوم برای شناسایی مناطقی از ژنوم که در گاومیش‌های خوزستانی و مازندرانی هدف انتخاب‌های طبیعی یا مصنوعی قرار گرفته‌اند، اجرا شده است. بدین منظور 148 رأس گاومیش رودخانه‌ای شامل جمعیت‌های خوزستانی (121 رأس) و مازندرانی (27 رأس)، به وسیلة آرایه‌های ژنومیکی Axiom® Buffalo Genotyping 90K تعیین ژنوتیپ شدند. جهت جستجوی نشانه‌های انتخاب از برآوردگر نااُریب FST (θ) استفاده شد. در مجموع 23 منطقه که نشانگرهای SNP آن‌ها بالاتر از 1/0 درصد حد بالای توزیع تجربی FST بود، به‌عنوان نشانه‌های انتخاب شناسایی شده و مورد بررسی‌های بیشتر قرار گرفتند. بعد از انطباق مناطق ژنومی انتخاب‌شده با مناطق ژنومی متناظر آن روی ژنوم گاو (UMD3.1 Bos Taurus Genome)، 64 ژن و 27 QTL شناسایی شد. تعدادی از این ژن‌ها در مطالعات قبلی نیز به‌عنوان نشانه‌های انتخاب گزارش شده‌اند. برخی از این ژن‌ها احتمالاً در مسیرهای بیولوژیکی که با اهلی‌شدن حیوانات (توسعة مغز و عملکردهای رفتاری، پیگماسیون و آداپته‌شدن به محیط زندگی و شرایط جغرافیایی) و تولید شیر مرتبط می‌باشند، درگیرند. بررسی‌ها همچنین نشان داد که QTLهای شناسایی‌شده در این تحقیق با صفات مهم اقتصادی از جمله صفات مرتبط با راندمان تبدیل غذایی، وزن بدن، چربی زیرپوستی، افزایش وزن روزانه، تیپ، تردی گوشت، ترکیبات شیر، اتصالات پستانی، اندازة گوساله و گوساله‌زایی آسان ارتباط دارند. به هرحال جهت شناسایی نقش دقیق این ژن‌ها و QTLها لازم است مطالعات پیوستگی و عملکردی بیشتری انجام گیرد.
کلیدواژه‌ها
پویش ژنوم؛ شاخص تمایز جمعیتی؛ گاومیش خوزستانی و مازندرانی؛ نشانه‌های انتخاب
عنوان مقاله [English]
Genome-Wide Survey of signature of positive selection in Khuzestani and Mazandrani buffalo breeds
نویسندگان [English]
Mahdi Mokhber1؛ Mohammad Moradi Shahrbabak2؛ Mostafa Sadeghi3؛ Hossein Moradi Shahrbabak3؛ John Williams4
1Ph.D Student, Department of Animal Science, Faculty of Agricultural Science and Engineering, University College of Agriculture and Natural Resources (UTCAN), University of Tehran, Karaj, Iran
2Department of Animal Science, Faculty of Agricultural Science and Engineering, University College of Agriculture and Natural Resources (UTCAN), University of Tehran, Karaj, Iran
3Department of Animal Science, Faculty of Agricultural Science and Engineering, University College of Agriculture and Natural Resources (UTCAN), University of Tehran, Karaj, Iran
4Parco Tecnologico Padano, Lodi, Italy,
چکیده [English]
Identification of selection targeted genomic regions is one of the most challenging areas of research in animal genetics, particularly in livestock. We carried out a genome-wide scan for signatures of positive selection to identify genomic regions that had been under selection in Iranian Khuzestani and Mazandrani buffalo breeds. A total of 148 water buffalo from Khuzestani (N=121) and Mazandrani (N=27) buffalo breeds were genotyped using Axiom® Buffalo Genotyping 90K Array. Unbiased method of population differentiation index (FST) was applied to detect signatures of selection. In total, 23 regions exceeding the 0.1 percent threshold of the empirical posterior distribution were identified as extremely differentiated. These selected genomic regions were surveyed to find encoding putative candidate genes and 64 genes and 27 QTL were extracted from the corresponding areas in UMD3.1 Bos Taurus Genome Assembly. Some of these genes have previously reported as signature of positive selection in the last studies. Some of these genes were also found to be involved in milk production traits and domestication-related changes include sensory perceptions, brain and neural system development, pigmentation, and geographic adaptation. Also, survey on extracted QTLs was shown that these QTLs involved in some economicl important traits in buffalo such as feed conversion ratio, subcutaneous fat, body weight, average daily gain, type, Meat tenderness, milk production conentent, udder attachment, calf size and calving ease traits. However, it will be necessary to carry out more association and functional studies to demonstrate the implication of these genes.
کلیدواژه‌ها [English]
signatures of selection, Genome-Wide Survey, population differentiation index, Khuzestani and Mazandrani buffalo breeds
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