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Optimization of alkaline protease production from a locally isolated Bacillus sp. ZR-5: Potential application as a detergent additive | ||
Progress in Biological Sciences | ||
مقاله 12، دوره 5، شماره 2، مهر 2015، صفحه 287-301 اصل مقاله (1.29 M) | ||
نوع مقاله: Original Research Papers | ||
شناسه دیجیتال (DOI): 10.22059/pbs.2015.56045 | ||
نویسندگان | ||
Hossein Mohammadian1؛ Mohammad Rabbani* 1؛ Ali Hafizi2؛ Hamid MirMohammad Sadeghi1؛ Amin Davary1؛ Fatemeh Moazen1؛ Ziaedin Samsam Shariat3؛ Zahra Etemadifar4 | ||
1Department of Pharmaceutical Biotechnology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. | ||
2Chemical Engineering Department, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz 71345, Iran | ||
3Department of Biochemistry, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran. | ||
4Department of Biology, School of Basic Sciences, University of Isfahan, Isfahan, Iran | ||
چکیده | ||
The aim of this study was to optimize protease production using a novel Bacillus sp. ZR-5 strain isolated from the soil, and evaluate its application in detergent industry. The proteolytic activity of the strain was demonstrated using gelatin hydrolysis screening test. Protease production optimization was carried out using a two-step approach: a conventional method in order to identify the best carbon and nitrogen sources followed by the application of response surface methodology (RSM) to optimize the factors, which include temperature, pH and incubation time. Glucose or fructose (5 g/L), wheat bran (5 g/L), temperatures of 25 and 55°C, pH 10.0 and an approximate incubation time of 44 h, were determined as the optimal conditions according to optimization processes. Validation tests were carried out under these conditions and the results were in good agreement with RSM predicted data. The in-gel activity (zymogram) test showed two hydrolytic zones with 66.2 and 36.5 kDa molecular weight on the casein containing polyacrylamide gel. The high compatibility in the presence of detergent powder and washing performance test suggested that the crude enzyme could be an appropriate choice as a detergent additive in detergent industries. | ||
کلیدواژهها | ||
alkaline proteases؛ Bacillus sp. ZR-5؛ detergent compatibility؛ media optimization؛ Response Surface Methodology | ||
مراجع | ||
1. Gupta, R., Beg, Q.K. and Lorenz, P. (2002). Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biot 59, 15-32. 2. Masui, A., Yasuda, M., Fujiwara, N. and Ishikawa, H. (2008). Enzymatic hydrolysis of gelatin layers on used lith film using thermostable alkaline protease for recovery of silver and PET film. Biotechnol. Progr, 20, 1267-1269. 3. Demain, A.L. and Adrio, J.L. (2008). In Natural Compounds as Drugs Volume I. Springer, in press., pp. 251-289. 4. Rao, M.B., Tanksale, A.M., Ghatge, M.S. and Deshpande, V.V. (1998). Molecular and biotechnological aspects of microbial proteases. Microbiol. Mol. Biol. R 62, 597-635. 5. Joo, H.S., Kumar, C.G., Park, G.C., Kim, K.T., Paik, S.R. and Chang, C.S. (2002). Optimization of the production of an extracellular alkaline protease from Bacillus horikoshii. Process. Biochem, 38, 155-159. 6. Kumar, C.G. and Takagi, H. (1999). Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol. Adv, 17, 561-594. 7. Wang, Q., Hou, Y., Xu, Z., Miao, J. and Li, G. (2008). Optimization of cold-active protease production by the psychrophilic bacterium Colwellia sp. NJ341 with response surface methodology. Bioresource. Technol, 99, 1926-1931. 8. Armstrong, N.A. (2006). Pharmaceutical experimental design and interpretation. CRC Press, London. 9. Singh, S.K., Singh, S.K., Tripathi, V.R., Khare, S.K. and Garg, S.K. (2011). Comparative onefactor- at-a-time, response surface (statistical) and bench-scale bioreactor level optimization of thermoalkaline protease production from a psychrotrophic Pseudomonas putida SKG-1 isolate. Microb. Cell. Fact, 10, 114. 10. Liu, S., Fang, Y., Lv, M., Wang, S. and Chen, L. (2010). Optimization of the production of organic solvent-stable protease by Bacillus sphaericus DS11 with response surface methodology. Bioresource. Technol, 101, 7924-7929. 11. Oskouie, S.F.G., Tabandeh, F., Yakhchali, B. and Eftekhar, F. (2008). Response surface optimization of medium composition for alkaline protease production by Bacillus clausii. Biochem. Eng. J, 39, 37-42. 12. Pillai, P., Mandge, S. and Archana, G. (2011). Statistical optimization of production and tannery applications of a keratinolytic serine protease from Bacillus subtilis P13. Process. Biochem, 46, 1110-1117. 13. Rao, Y.K., Lu, S.-C., Liu, B.-L. and Tzeng, Y.-M. (2006). Enhanced production of an extracellular protease from Beauveria bassiana by optimization of cultivation processes. Biochem. Eng. J 28, 57-66. 14. Reddy, L., Wee, Y.-J., Yun, J.-S. and Ryu, H.-W. (2008). Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett–Burman and response surface methodological approaches. Bioresource. Technol, 99, 2242-2249. 15. Thys, R., Guzzon, S.O., Cladera-Olivera, F. and Brandelli, A. (2006). Optimization of protease production by Microbacterium sp. in feather meal using response surface methodology. Process. Biochem, 41, 67-73. 16. Fakhfakh-Zouari, N., Haddar, A., Hmidet, N., Frikha, F. and Nasri, M. (2010). Application of statistical experimental design for optimization of keratinases production by Bacillus pumilus A1 grown on chicken feather and some biochemical properties. Process. Biochem, 45, 617-626. 17. Haddar, A., Fakhfakh-Zouari, N., Hmidet, N., Frikha, F., Nasri, M. and Kamoun, A.S. (2010). Low-cost fermentation medium for alkaline protease production by Bacillus mojavensis A21 using hulled grain of wheat and sardinella peptone. J. Biosci. Bioeng 110, 288-294. 18. Rabbani, M., Bagherinejad, M.R., Sadeghi, H.M., Shariat, Z.S., Etemadifar, Z., Moazen, F., Rahbari, M., Mafakher, L. and Zaghian, S. (2013). Isolation and characterization of novel thermophilic lipase-secreting bacteria. Braz. J. Microbiol 44, 1113-1119. 19. Mukherjee. A. K and K., R.S. (2011). A statistical approach for the enhanced production of alkaline protease showing fibrinolytic activity from a newly isolated Gram-negative Bacillus sp. strain AS-S20-I. New. Biotechnol, 28, 182-189. 20. Garciacarreno, F., Dimes, L. and Haard, N. (1993). Substrate-gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors. Anal. Biochem, 214, 65-69. 21. Arulmani, M., Aparanjini, K., Vasanthi, K., Arumugam, P., Arivuchelvi, M. and Kalaichelvan, P.T. (2007). Purification and partial characterization of serine protease from thermostable alkalophilic Bacillus laterosporus-AK1. World. J. Microb. Biot 23, 475-481. 22. Hübner, U., Bock, U. and Schügerl, K. (1993). Production of alkaline serine protease subtilisin Carlsberg by Bacillus licheniformis on complex medium in a stirred tank reactor. Appl. Microbiol. Biotechnol., 40, 182-188. 23. Maurer, K.-H. (2004). Detergent proteases. Curr. Opin. Biotech 15, 330-334. 24. Rai, S.K. and Mukherjee, A.K. (2011). Optimization of production of an oxidant and detergentstable alkaline β-keratinase from Brevibacillus sp. strain AS-S10-II: Application of enzyme in laundry detergent formulations and in leather industry. Biochem. Eng. J 54, 47-56. 25. Kumar, C. (2002). Purification and characterization of a thermostable alkaline protease from alkalophilic Bacillus pumilus. Lett. Appl. Microbiol 34, 13-17. 26. Huang, Q., Peng, Y., Li, X., Wang, H. and Zhang, Y. (2003). Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus. Curr. Microbiol, 46, 0169-0173. 27. Miyaji, T., Otta, Y., Nakagawa, T., Watanabe, T., Niimura, Y. and Tomizuka, N. (2006). Purification and molecular characterization of subtilisin‐like alkaline protease BPP‐A from Bacillus pumilus strain MS‐1. Lett. Appl. Microbiol, 42, 242-247. | ||
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