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پیامد رسوبگذاری زیستی کربنات کلسیم بر آبشویی باکتری اشریشیا کولی در ستون شنی | ||
| تحقیقات آب و خاک ایران | ||
| مقاله 18، دوره 50، شماره 10، اسفند 1398، صفحه 2619-2631 اصل مقاله (1.06 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2019.281919.668210 | ||
| نویسندگان | ||
| ریحانه شوکتی1؛ نسرین قربان زاده* 2؛ محمدباقر فرهنگی3؛ محمود شعبانپور شهرستانی4 | ||
| 1علوم و مهندسی خاک، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 2گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 3گروه علوم و مهندسی خاک، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران | ||
| 4دانشیار گروه خاکشناسى، دانشکده کشاورزى، دانشگاه گیلان، رشت | ||
| چکیده | ||
| پیشبینی انتقال باکتریها به آبهای زیرزمینی برای جلوگیری از آلودگی منابع آب به باکتریهای کلیفرم بسیار حائز اهمیت است. این پژوهش با هدف بررسی کارایی روش رسوبگذاری زیستی کربنات کلسیم (MICCP) در جلوگیری از انتقال باکتری اشریشیا کولی به عنوان باکتری شناساگر آلودگی زیستی انجام شده است. برای انجام فرایند MICCP نمونه شن در لوله PVC (قطر داخلی 8/4 و بلندی 92/14 سانتیمتر) ریخته شد و برای سه روز همراه با باکتری اسپوروسارسینا پاستئوری انکوباسیون شد. پس از پیدایش جریان ماندگار اشباع، به اندازه 1/0 حجم منفذی از سوسپانسیون باکتری اشریشیا کولی (108 CFU/mL) به ستون افزوده شد و آبشویی با آب مقطر انجام شد. از زهآب خروجی از ستونها در حجمهای منفذی 1/0 تا 5 نمونهبرداری شد. پس از پایان آبشویی، ستون شن به 5 برش 3 سانتیمتری تقسیم شد و شمار باکتریهای به دام افتاده در ستون اندازهگیری شدند. شمارش باکتریها در محیط کشت EMB و به روش شمارش کلنی انجام شد. ستون شن کنترل نیز آبشویی شد. پیامد تیمار MICCP و برهمکنش آن با حجم منفذی بر شمار اشریشیا کولی در زهآب معنیدار شد (05/0>p). پیامد عمق و برهمکنش آن با تیمار نیز بر شمار مانده باکتری اشریشیا کولی در ستون و کربنات کلسیم معنیدار بود (05/0>p). رویهمرفته، کارکرد کربنات کلسیم فراهم شده زیستی در پالایش باکتریها به ویژه در لایههای بالایی ستون قابل توجه بوده و با کاهش ضریب آبگذری ستون شن، غلظت باکتری اشریشیا کولی در زهآب کاهش یافت. | ||
| کلیدواژه‌ها | ||
| کانی‌شدن زیستی؛ اوره‌آز؛ ضریب آبگذری؛ اسپوروسارسینا پاستئوری | ||
| عنوان مقاله [English] | ||
| The Effect of Calcium Carbonate Bioprecipitation on Escherichia coli Leaching in Sand Column | ||
| نویسندگان [English] | ||
| Reyhaneh Shokati1؛ Nasrin Ghorbanzadeh2؛ Mohammad Bagher Farhangi3؛ Mahmoud Shabanpour4 | ||
| 1Department of Soil Science, University of Guilan, Rasht, Iran | ||
| 2Department of soil Science, University of Guilan, Rasht, Iran | ||
| 3Department of Soil Science, University of Guilan, Rasht, Iran | ||
| 4Associate Professor, Department of Soil Science, University of Guilan, Rasht, Iran | ||
| چکیده [English] | ||
| Prediction of bacteria transport to groundwater is very important to prevent water resources from coliform bacteria pollusion. The objective of this study was to investigate the effectiveness of microbially induced calcium carbonate precipitation (MICCP) in preventing the transport of Escherichia coli as an indicator coliform. For the MICCP process, the sand sample was poured into PVC tubes (with inside diameter of 4.8 and height of 14.92 cm) and incubated for 3 days in the presence of Sporosarcina pasteurii. After the stablishment of steady-state flow, 0.1 pore volume of Escherichia coli suspension (108 CFU/mL) was added to the sand column and the leaching was followed with distilled water. The column effluent was sampled in 0.1 to 5 pore volume. After leaching, the sand column was sliced into five sections (~3 cm), and the number of E. coli trapped in each slice was measured using the plate count method in EMB agar culture medium. Leaching was also carried out in control sand column. The effect of MICCP treatment and its interaction with pore volume was significant (p<0.05) on Escherichia coli count in effluent. The effect of depth and its interaction with MICCP treatment were significant (p<0.05) on E. coli residuals and bioprecipitated calcium carbonate in the column. Overall, the role of biopreciptated calcium carbonate was significant in bacteria filtration, as it decreased the sand column hydraulic conductivity and reduced the number of E. coli in column effluents. | ||
| کلیدواژه‌ها [English] | ||
| Biomineralization, Urease, Hydraulic conductivity, Sporosarcina pasteurii | ||
| مراجع | ||
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