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برهمکنش سینرژیستی قارچ Beauveria bassiana و دو فرمولاسیون تجاری خاک دیاتومه روی لمبه گندم، Trogoderma granarium (Coleoptera: Dermestidae) | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| دوره 10، شماره 1، خرداد 1400، صفحه 63-76 اصل مقاله (901.36 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2022.337512.316 | ||
| نویسندگان | ||
| افسانه شهبازی1؛ مرضیه علیزاده* 2؛ حمیدرضا پوریان2 | ||
| 1دانش آموخته کارشناسی ارشد گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
| 2استادیار گروه گیاهپزشکی، دانشکده کشاورزی ، دانشگاه رازی، کرمانشاه، ایران | ||
| چکیده | ||
| عوامل حفاظت زیستی شامل عوامل زنده بیوکنترل و مواد غیرزنده با پایه طبیعی نظیر خاک دیاتومه، در کنترل آفات انباری، نقش مهمی را به‌لحاظ حفظ سلامت غذایی ایفا می‌کنند. مشکل کاربرد خاک‌های دیاتومه در انبار این است که دوز توصیه شده آنها سبب افزایش توده‌ای شدن و کاهش جریان‌پذیری بذرها می‌شود. بنابراین پژوهش حاضر با هدف تعیین سازگاری زیستی بین دو فرمولاسیون تجاری خاک دیاتومه ایرانی Sayan و خارجی Celite 610 با جدایه بومی قارچ بیمارگر حشرات، Beauveria bassiana DE (Hypocreales) و نیز رفع مشکل کاهش جریان‌پذیری بذرها با استفاده از دوز کم‌کشنده خاک دیاتومه در مخلوط انجام شد. به‌این منظور، ابتدا آزمون‌های زیست‌سنجی 14 روزه به-روش آغشته کردن بذور گندم به‌طور جداگانه با قارچ و دو خاک دیاتومه علیه حشرات بالغ لمبه گندم،Trogoderma granarium Everts ، انجام شد. سپس به‌منظور تعیین برهمکنش، پنج دوز از قارچB. bassiana با LD25 هر یک از خاک‌های دیاتومه مخلوط شدند. طبق نتایج مقادیر LD50 برای قارچ، سایان® و سلایت 610® به‌ترتیب، 3/295، 5/4439 و 3/992 پی‌پی‌ام محاسبه شدند. بر اساس آزمون نسبت دوزهای کشنده مشخص شد که قدرت کشندگی سلایت 610®، 47/4 برابر سایان® است. همچنین برهمکنش در تمام مخلوط‌های حاصل از قارچ و دوز کم‌کشنده هر یک از خاک‌های دیاتومه، سینرژیستی بود به‌جز بالاترین دوز که از نوع افزایشی تخمین زده شد. همچنین کلیه دوزهای اختلاط بر کاهش تولید نتاج، طی دوره ذخیره سازی هشت هفته‌ای موثر واقع شدند که بیانگر سازگاری این دو عامل برای کاربرد هم‌زمان در برنامه‌های مدیریت تلفیقی T. granarium می‌باشد. | ||
| کلیدواژه‌ها | ||
| اختلاط؛ قارچ‌های بیمارگر حشرات؛ سایان®؛ سلایت 610®؛ بیوکنترل | ||
| عنوان مقاله [English] | ||
| Synergistic interaction of Beauveria bassiana and two commercial formulations of diatomaceous earth on Trogoderma granarium (Coleoptera: Dermestidae) | ||
| نویسندگان [English] | ||
| Afsaneh Shabazi1؛ Marzieh Alizadeh2؛ Hamid-Reza Pourian2 | ||
| 1Former M.SC. Student, Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran. | ||
| 2Assistant Professor, Department of Plant Protection, Faculty of Agriculture, Razi University, Kermanshah, Iran | ||
| چکیده [English] | ||
| Bioprotection agents, including living biocontrol agents and naturally occurring non-living substances such as diatomaceous earth, play a crucial role in controlling stored commodity pests to maintain food health. The problem with using diatomaceous earth in storage is that their recommended dose increases grains' bulk density and decreases flowability. Therefore, the present study aimed to determine the biocompatibility between two commercial formulations of Iranian diatomaceous earth, Sayan, and foreign Celite 610 with the indigenous entomopathogenic fungus isolate, Beauveria bassiana DE (Hypocreales), and to solve the problem of reducing grains' flowability using a low-lethal dose of diatomaceous earth in the mixture. For this purpose, 14-day bioassays were first performed by impregnating wheat seeds separately with the fungus and two diatomaceous earths against adults of Khapra beetle, Trogoderma granarium Everts. Then, to determine the interaction effects, five different doses of B. bassiana were mixed with LD25 of each diatomaceous earth. Based on the results, LD50 values for fungus, Sayan®, and Celite 610® were calculated at 295.3, 4439.5, and 992.3 ppm, respectively. According to the lethal dose ratio test, the insecticidal activity of Celite 610® was estimated to be 4.47 times more than Sayan®. Moreover, the interaction was synergistic in all mixtures of B. bassiana with the low-lethal dose of each diatomaceous earth except for the highest dose of the fungus, which was estimated additive. In addition, all mixtures effectively reduced progeny production during the eight-week storage period, indicating the compatibility of these two agents for co-application in the integrated T. granarium management programs. | ||
| کلیدواژه‌ها [English] | ||
| combination, entomopathogenic fungi, Sayan®, Celite 610®, biocontrol | ||
| مراجع | ||
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