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اثر دما و طول دوره‌ی سرما بر گل‌انگیزی سوخ‌های دو رقم پیاز خوراکی بومی ایران | ||
| علوم باغبانی ایران | ||
| دوره 54، شماره 1، فروردین 1402، صفحه 105-116 اصل مقاله (1.02 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijhs.2022.340368.2011 | ||
| نویسندگان | ||
| ناهید مرادی معتقد؛ فرشاد دشتی* ؛ فهیمه قائمی‌زاده | ||
| گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه بوعلی سینا، همدان، ایران | ||
| چکیده | ||
| پیاز گیاهی دو ساله است که در سال اول در پاسخ به طول روز بلند بهار یا تابستان تولید سوخ می‌کند و سپس با گذراندن زمستان و دریافت سرما در سال دوم (بهارش ) از مواد غذایی ذخیره شده در سوخ، جهت تولید ساقه گل‌دهنده و اندام زایشی استفاده می‌کند (Lee et al., 2013). دمای مناسب بهارش و همچنین مدت زمان مواجهه با آن، بسته به رقم متفاوت است و اگر به درستی اعمال نشود گلدهی به خوبی صورت نخواهد گرفت. اطلاع از فاکتورهای کنترل آغازش و توسعه‌ی گل‌آذین می‌تواند در تکنیک‌هایی برای مهار گلدهی و یا تحریک گلدهی جهت کارهای اصلاحی و تولید بذر مورد استفاده قرار گیرد. از آن‌جایی‌که تا کنون مطالعه‌ای در زمینه‌ی نیاز سرمایی بهارش در ارقام پیاز بومی ایران صورت نگرفته است، مطالعه‌ی حاضر با هدف تعیین مدت زمان و دمای مناسب بهارش در دو رقم پیاز خوراکی بومی ایران و همچنین بررسی الگوی بیان ژن AcFT2 انجام گرفته است. | ||
| کلیدواژه‌ها | ||
| بهارش؛ گل انگیزی؛ ریل تایم پی‌سی‌آر؛ SEM؛ AcFT2 | ||
| عنوان مقاله [English] | ||
| Effect of Temperature and Cold Storage Durations on Flower Induction in Bulbs of Two Iranian Onion Cultivars | ||
| نویسندگان [English] | ||
| Nahid Moradi Motaghed؛ Farshad Dashti؛ Fahimeh Ghaemizadeh | ||
| Department of Horticultural Science, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran | ||
| چکیده [English] | ||
| In order to determine cold requirements for flower induction (vernalization), the adult bulbs of Ghermez Azarshahr and Sefid Qom onion cultivars were studied in four time periods (two, four, six and eight weeks) and three temperatures (5, 10 and 15 °C) in a factorial experiment based on a completely randomized design with three replicates. Scaning electron microscope (SEM) was used to determine swelling of reproductive meristem (meristem dome) after applying the treatments. Also, the temporal expression patterns of flowering integrator gene (AcFT2) were analyzed in cv; Ghermez Azarshahr, using Real-Time PCR. According to obtained results, the maximum meristem inflation of Sefid Ghom was observed in 10 and 15 °C, while in Ghermez Azarshahr at 5 °C. Bulbs showed the lowest meristem inflation (30.53%) after 2 weeks of cold storage, and with increasing vernalization time, up to 6 weeks, the meristem inflation (75.35%) increased. The highest relative expression of AcFT2 was observed in the Ghermez Azarshahr after 6 weeks of cold storage at 5 °C, which coincides with meristem inflation results. Ultimately, the best vernalization condition were determined as 6 weeks cold storage at 10 ° or 15 °C for Sefid Ghom and 5 °C for Ghermez Azarshahr. | ||
| کلیدواژه‌ها [English] | ||
| AcFT2, Flower induction, Real-Time PCR, SEM, Vernalization | ||
| مراجع | ||
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