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ویژگیهای بیولوژیکی و مولکولی چهار جدایه ویروس وای سیبزمینی (PVY) براساس بخشی از ناحیه ژنی رمزکننده پروتئین پوششی | ||
دانش گیاهپزشکی ایران | ||
دوره 54، شماره 2، اسفند 1402، صفحه 349-371 اصل مقاله (2.91 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijpps.2024.371771.1007053 | ||
نویسندگان | ||
پرستو پورشریفی1؛ اکبر دیزجی* 2؛ پریسا شریفی نظام آباد3؛ مینا کوهی حبیبی4؛ هادی خاطری5 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران. | ||
3بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، گرگان، ایران. | ||
4گروه گیاهپزشکی دانشکده کشاورزی دانشگاه تهران. کرج. ایران | ||
5عضو هیأت علمی گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
چکیده | ||
ویروس وای سیبزمینی (Potato virus Y, PVY) یکی از ویروسهای محدودکننده تولید توتون و سیبزمینی در دنیا میباشد. به منظور بررسی خصوصیات بیولوژیکی و مولکولی جدایههای میزبانی ویروس وای سیبزمینی، طی سالهای 1389 و 1390، در مجموع 1178 نمونه از گیاهان توتون، سیبزمینی، فلفل و علف هرز عروسک پشت پرده که دارای علائم موزائیک بودند از هشت شهرستان مختلف کشور شامل کرج، ارومیه، رشت، سنندج، شیراز، قزوین، ورامین و همدان جمعآوری شد. آلودگی نمونهها به PVY با آزمون سرولوژیکی ساندویچ دو طرفه الیزا (double antibody sandwich enzyme-linked immunosorbent assay, DAS-ELISA) بررسی و میزان آلودگی در بین نمونههای توتون، سیبزمینی، عروسکپشتپرده و فلفل به ترتیب 2/27، 4/20، 75/18 و صفر درصد برآورد گردید. دامنه میزبانی آزمایشی چهار جدایه منتخب ویروس وای سیبزمینی (Po2، Po5، Tob2 و Ph1) روی 12 گونه گیاه محک در شرایط گلخانه تفاوتهای جزئی داشت. پس از تکثیر انتهای 3' ژنوم جدایهها به اندازه 1200 جفت باز، درخت تبارزائی براساس توالی نوکلئوتیدی یک سوم بالادست ناحیه ژنی رمزکننده پروتئین پوششی بازسازی شد که جدایههای Tob2، Ph1 و Po5 در کلاد PVYN/NTN و جدایه Po2 در کلاد PVYO قرار گرفتند. نتایج IC-RT-PCR با استفاده از آغازگرهای اختصاصی سویه، و مقایسه توالیهای نوکلئوتیدی و بررسی موتیفهای اختصاصی سویه در همردیفهای توالی آمینواسیدی پیش بینی شده انتهای آمینی پروتئین پوششی، نتایج واکاوی تبارزائی را تایید کردند. این اولین گزارش از آلودگی گونه Physalis divaricata به سویه PVYN/NTN در دنیا میباشد. | ||
کلیدواژهها | ||
تبارزائی؛ سویه؛ سرولوژی؛ عروسک پشت پرده | ||
عنوان مقاله [English] | ||
Biological and molecular characteristics of four potato virus Y (PVY) isolates based on partial coat protein gene | ||
نویسندگان [English] | ||
Prastoo Poursharifi1؛ Akbar Dizadji2؛ Parisa sharifinezamabad3؛ Mina Koohi Habibi4؛ Hadi Khateri5 | ||
1Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
2Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran. | ||
3Department of Plant Protection, Agricultural and Natural Resources and Research Center of Golestan province, Gorgan, Iran. | ||
4Department of Herbal Medicine, Faculty of Agriculture, University of Tehran. Karaj. Iran | ||
5Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran | ||
چکیده [English] | ||
Potato virus Y (PVY) is one of the viruses limiting tobacco and potato production in the world. To investigate the biological and molecular characteristics of PVY isolates, during 2010-2011, a total of 1178 symptomatic leaf samples of tobacco, potato, pepper and grand cherry (Physalis divaricata) plants were collected from eight different cities including Karaj, Urmia, Sanandaj, Shiraz, Qazvin, Varamin and Hamedan of Iran. The samples were tested serologically by double-antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISA) and PVY was detected in tobacco (27.27 %), potato (20.4 %) and grand cherry (18.75 %) samples, with no infection in pepper samples. Four biologically purified PVY isolates (Po2, Po5, Tob2 and Ph1) revealed few differences in experimental host range studies among 12 test plant species. After amplification of the 3´ genomic end of four PVY isolates with the expected size of 1200 bp, phylogenetic analysis based on the nucleotide sequences of partial coat protein gene (encoding N- terminal region of coat protein) revealed that Tob2, Po5 and Ph1 isolates grouped into PVYN/NTN clade, while only isolate Po2 grouped into PVYO clade. The results of IC-RT-PCR using strain-specific primers and further pairwise nucleotide sequence comparisons and strain-specific motifs analysis in the predicted amino acid sequences of the N-terminal region of coat protein confirmed the results of phylogenetic analysis. This is the first report of Physalis divaricata infection with PVYN/NTN strain in the world. | ||
کلیدواژهها [English] | ||
Phylogeny, strain, serology, Physalis | ||
مراجع | ||
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