An amperometric hand-held Nitrate biosensor based on Nitrate reductase immobilized on a thin film electrode: a tool for in-filed water and food analysis | ||
| Journal of Food and Bioprocess Engineering | ||
| دوره 7، شماره 1، آبان 2024، صفحه 52-57 اصل مقاله (1.4 M) | ||
| نوع مقاله: Original research | ||
| شناسه دیجیتال (DOI): 10.22059/jfabe.2024.373543.1167 | ||
| نویسندگان | ||
| MOHAMAD Savarolyia* 1؛ Hossein Kiani1؛ Mohammad Mousavi2 | ||
| 1Bioprocessing and Biodetection Lab (BBL), Department of Food Science, Technology, and Engineering, University of Tehran, Karaj, Iran. | ||
| 2Bioprocessing and Biodetection Lab (BBL), Department of Food Science, Technology, and Engineering, University of Tehran, Karaj, Iran | ||
| چکیده | ||
| This study presents the design and evaluation of a portable amperometric biosensor for detecting nitrates, utilizing a gold three-electrode system with immobilized nitrate reductase. With a sensitivity of 12.01 μA mM−1 cm−2 over a 0.16–0.96 mM range and a regression coefficient of 0.98, the biosensor outperforms traditional UV spectrophotometric methods (0.01–0.19 mM) by offering a broader range (0.16–0.96 mM) and greater accuracy, as evidenced by a 6.69% relative standard deviation for reproducibility and a detection limit of 0.14 mM. Maintaining 63% of its initial response after four days and showing minimal interference from nitrite (1 mM), this biosensor is highly suitable for water testing, although pre-treatment is required for analyzing food samples due to phenolic compounds. | ||
| کلیدواژهها | ||
| Nitrate؛ Biosensor؛ Amperometric؛ Enzyme؛ Immobilization | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 579 تعداد دریافت فایل اصل مقاله: 462 |
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